Method for detecting polynucleotides with immobilized polynucleo

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

536 243, 536 2431, 536 2432, C12Q 168, C07H 2104

Patent

active

056396120

ABSTRACT:
A method for detecting the presence of a particular organism, infectious agent, or biological component of a cell or organism in a sample, based on sandwich hybridization in which first and second probes are used, and the specificity of the first probe is determined based on its melting temperature (T.sub.m) with the target polynucleotide at a selected sodium and formamide concentration.

REFERENCES:
patent: 4729950 (1988-03-01), Kricka et al.
patent: 4734363 (1988-03-01), Dattagupta et al.
patent: 4751177 (1988-06-01), Stabinsky
patent: 4797355 (1989-01-01), Stabinsky
patent: 4851330 (1989-07-01), Kohne
patent: 4894325 (1990-01-01), Englehardt et al.
patent: 5081584 (1992-01-01), Omichinski et al.
patent: 5082935 (1992-01-01), Cruickshank
patent: 5084565 (1992-01-01), Parodos et al.
Meinkoth et al., Analyt. Bioch. 138, 267-284 (1984).
L. J. Emorine et al., "Structure of the gene for human .beta..sub.2 -adrenergic receptor: Expression and promoter characterization", Proceedings of the National Academy of Science, USA, vol. 84, Oct. 1987, pp. 6995-6999.
C. B. Harley, "Hybridization of Oligo(dT) to RNA on Nitrocellulose", Gene Analysis Techniques, vol. 4, 1987, pp. 17-22.
A. R. Dunn et al., "Mapping Viral mRNAs by Sandwich Hybridization", Methods in Enzymology, vol. 65, pt. 1, 1980, pp. 468-478.
A. Palva et al., "Laboratory Methods: Quantification of .alpha.-Amylase mRNA in Bacillus subtilis by Nucleic Acid Sandwich Hybridization", DNA, vol. 7, No. 2, 1988, pp. 135-142.
R. Nussinov, "Efficient algorithms for searching for exact repetition of nucleotid sequences.", Journal of Molecular Evolution, vol. 19, Nos. 3-4, pp. 283-285.
W. W. Ralph et al., "A modified Chou and Fasman protein structure algorithm", Computer Application in the Biosciences, vol. 8, No. 3, Sep. 1987, pp. 211-216.
C. Hough et al., "Differential down-regulation of .beta..sub.1 -and .beta..sub.2 -adrenergic receptor mRNA in C.sub.6 glioma cells", Biochemical and Biophysical Research Communications, vol. 170, No. 1, 1990, pp. 46-52.
A. Ballagi-Pordany et al., "Quantitative determination of mRNA phenotypes by the polymerase chain reaction", Analytical Biochemistry, vol. 196, 1991, pp. 89-94.
B. Feve et al., "Differential regulation of .beta..sub.1 -and .beta..sub.2 -adrenergic receptor protein and mRNA levels by glucocorticoids during 3T3-F442A adipose differentiation", Journal of Biological Chemistry, vol. 265, No. 27, Sep. 25, 1990, pp. 16343-16349.
P. Muzzin et al., "An adipose tissue-specific .beta.-adrenergic receptor", Journal of Biological Chemistry, vol. 266 No. 35, Dec. 15, 1991, pp. 24053-24058.
C. Albretsen et al., "Optimal conditions for hybridization with oligonucleotides: a study with myc -oncogene DNA probes", Analytical Biochemistry, vol. 170, 1988, pp. 193-202.
M. S. Waterman et al., "Phase transitions in sequence matches and nucleic acid structure", Proceedings of the National Academy of Science USA, vol. 84, Mar. 1987, pp. 1239-1243.
M. S. Waterman et al., "A new algorithm for best subsequence alignments with application to tRNA-rRNA comparisons", Journal of Molecular Biology, vol. 197, 1987, pp. 723-728.
R. J. Cano et al., "DNA hybridization assay using ATTOPHOS.TM., a fluorescent substrate for alkaline phosphatase", Biotechniques, vol. 12, No. 2, Feb. 1992, pp. 264-269.
N. P. Gerard, "Human substance P Receptor (NK-1): Organization of the gene, chromosome localization, and functional expression of cDNA clones", Biochemistry, vol. 30, 1991, pp. 10640-10646.
S. -I. Hirai et al., "Characterization of junD: a new member of the jun protooncogene family", The EMBO Journal, vol. 8, No. 5, 1989, pp. 1433-1439.
D. T. Jones et al., "Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium", Journal of Biological Chemistry, vol. 262, No. 29, Oct. 15, 1987, pp. 14241-14249.
L. Li et al., "Different members of the jun protooncogene family exhibit distinct patterns of expression in response ot type .beta. transforming growth factor", Journal of Biological Chemistry, vol. 265, No. 3, Jan. 25, 1990, pp. 1556-1562.
Y. Yokota et al., "Molecular characterization of a functional cDNA for rat substance P receptor", Journal of Biological Chemistry, vol. 264, No. 30, Oct. 25, 1989, pp. 17649-17652.
J. Codina et al., ".alpha..sub.i 3 cDNA encodes the .alpha. subunit of G.sub.k, the stimulatory G protein of receptor-regulated K.sup.+ channels", Journal of Biological Chemistry, vol. 263, No. 14, May 15, 1988, pp. 6746-6750.
H. Itoh et al., "Presence of three distinct molecular species of G.sub.i protein .alpha. subunit", Journal of Biological Chemistry, vol. 263, No. 14, May 15, 1988, pp. 6656-6664.
K. Hattori et al., "Structure and chromosomal localization of the functional intronless human JUN protooncogene", Proceedings of the National Academy of Science USA, vol. 85, Dec. 1988, pp. 9148-9152.
P. Bray et al., "Human cDNA clones for an .alpha. subunit of G.sub.i signal-transduction proteins", Proceedings of the National Academy of Science USA, vol. 84, Aug. 1987, pp. 5115-5119.
C. R. Beals et al., "A small multigene family encodes G.sub.i signal-transduction proteins", Proceeding of the National Academy of Science USA, vol. 84, Nov. 1987, pp. 7886-7890.
B. A. Harris, "Complete cDNA sequence of a human stimulatory GTP-binding protein alpha subunit", Nucleic Acids Research, vol. 16, No. 8, 1988, p. 3585.
R. Mattera et al., "Identification by molecular cloning of two forms of the .alpha.-subunit of the human liver stimulatory (G.sub.s) regulatory component of adenylyl cyclase", FEBS Letters, vol. 206, No. 1, Sep. 1986, pp. 36-42.
J. R. Didsbury et al., "Molecular cloning of a new human G protein", FEBS Letters, vol. 219, No. 1, Jul. 1987, pp. 259-263.
A. Swaroop et al., "Differential expression of novel G.sub.s.alpha. signal transduction protein cDNA species", Nucleic Acids Research, vol. 19, No. 17, 1991, pp. 4725-4729.
Polsky-Cynkin et al., "Use of DNA Immobilized on Plastic and Agarose Supports to Detect DNA by Sandwich Hybridization", Clinical Chemistry, vol. 31, No. 9, 1985, pp. 1438-1443.
Hayashi et al., "A Novel Diagnostic Method of Pneymocystis carinii", Laboratory Investigation, vol. 63, No. 4, 1990, pp. 575-580.
Giovannoni et al., "Phylogenic Group-Specific Oligodeoxynucleotide Probes for Identification of Single Microbial Cells", Journal of Bacteriology, vol. 170, No. 2, Feb. 1988, pp. 720-726.
Kitabayashi et al., "Nucleotide Sequence of Rat c-jun Protooncogene", Nucleic Acids Research, vol. 18, No. 11, 1990, EMBL Accession No. X17215.
Hershey et al., "Molecular Characterization of a Functional cDNA Encoding the Rat Substance P Receptor", Science, vol. 247, Feb. 23, 1990, pp. 958-961.
Chung et al., "Cloning and Sequence Analysis of the Human Brain B-adrenergic Receptor", FEBS Letters, vol. 211, No. 2, pp. 200-206.
Saiki et al., "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase", Science, vol. 239, Jan. 29, 1988, pp. 487-491.
I. Raineri et al., "Improved Efficiency for Single-Sided PCR by Creating a Reusable Pool of First-Strand cDNA coupled to a Solid Phase", Nucleic Acids Research, vol. 19, No. 14, 1991, p. 4010.
S. Inouye et al., "Microplate Hybridization of Amplified Viral DNA Segment", Journal of Clinical Microbiology, vol. 28, No. 6, Jun. 1990, pp. 1469-1472.
M. J. Palazzolo et al., "A Family of Lambda Phage cDNA Cloning Vectors, .lambda.SWAJ, Allowing the Amplification of RNA Sequences", Gene, vol. 52, 1987, pp. 197-206.
R. Bischoff et al., "Introduction of 5'-Terminal Functional Groups into Synthetic Oligonucleotides for Selective Immobilization", Analytical Biochemistry, vol. 164, No. 2, Aug. 1, 1987, pp. 336-344.
I. Martin et al., "Characterization of the levanase gene of Bacillus subtilis which shows homology to yeast invertase", Mol. Gen. Genet., vol. 208, 1987, pp. 177-184.
D. Betzl et al., "Identification of Lactococci and Enterococci by Colony Hybridization with 23S rRNA-Targeted Oligonucleotide Probes", Applied and Environmental Microbiology, vol. 56, No. 9, 1990, pp. 2927-2929.
E. Baldwin et al., "Generation of a Catalytic An

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for detecting polynucleotides with immobilized polynucleo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for detecting polynucleotides with immobilized polynucleo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting polynucleotides with immobilized polynucleo will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2157016

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.