Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1986-04-16
1991-01-01
Yarbrough, Amelia Burgess
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 91, 435803, 436501, 436813, 536 27, 935 3, 935 9, 935 19, 935 29, 935 78, 935 80, C12Q 168, C12P 1934, G01N 3502, C12N 1503
Patent
active
049817833
ABSTRACT:
A method is disclosed for the analysis of gene expression in human biopsy that is useful in the diagnosis and prognosis of disease and evaluation of risk for disease. The method comprises the steps of compiling data regarding the level of expression of certain individual cloned gene sequences from patients having defined pathological conditions and from normal patients and thereafter identifying sequences that characterize the pathological condition.
The data regarding the expression of the individual cloned genes are stored in a defined pattern or array. Replicas of this array are hybridized to radioactive probes made using the RNA isolated from biopsy tissue. The extent of hybridization of the probe to each of the cloned sequences is proportional to the level of expression of the cloned sequence in the tissue from which the probe was made. This may be done by exposing the hybridized clones to x-ray film and scanning the x-ray films to quantify the cloned sequences. This is preferably done with a computer driven scanner. The digitized data may be processed using a computer program. The method may be used to determine the expression of cloned sequences in human colon biopsy samples of malignant of premalignant tissue as well as for monitoring the effects of chemotherapeutic agents.
An apparatus is also disclosed for use in carrying out an automated assay.
REFERENCES:
patent: 4699877 (1987-10-01), Cline et al.
Burgess Yarbrough Amelia
Casella Anthony J.
Hespos Gerald E.
Marschel Ardin H.
Montefiore Medical Center
LandOfFree
Method for detecting pathological conditions does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for detecting pathological conditions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting pathological conditions will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1995805