Method for detecting pathogens using molecular beacons

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S006120, C536S023100, C536S024320, C536S024330

Reexamination Certificate

active

07993881

ABSTRACT:
A method for detecting pathogens, particularly organisms associated with sexually transmitted diseases, especially Human papilloma virus genotypes is described. The method involves the use of real-time PCR using specially designed probes. The probes, kits for carrying out the method, and methods for designing primers suitable for use in the method of the invention are also described.

REFERENCES:
patent: 6037130 (2000-03-01), Tyagi et al.
patent: 6159729 (2000-12-01), Hofmann et al.
patent: 6713262 (2004-03-01), Gellibolian et al.
patent: 2002/0102571 (2002-08-01), Theaker et al.
patent: 2003/0068625 (2003-04-01), Sheehan et al.
patent: 2004/0151723 (2004-08-01), Maeda et al.
patent: 2005/0176023 (2005-08-01), Ramon et al.
patent: 2005/0215497 (2005-09-01), Harel-Bellan et al.
patent: 0477972 (1991-09-01), None
patent: 1201771 (2002-05-01), None
patent: WO 93/00435 (1993-01-01), None
patent: WO 01/09386 (2001-02-01), None
patent: WO 03/000933 (2003-01-01), None
patent: WO 03/057914 (2003-07-01), None
patent: WO 03/076667 (2003-09-01), None
patent: WO 2005/033333 (2005-04-01), None
El-Hajj et al. Detection of Rifampin Resistance in Mycobacterium tuberculosis in a Single Tube with Molecular Beacons. Journal of Clinical Microbiology (2001) 39(11): 4131-4137.
Vet et al. Multiplex detection of four pathogenic retroviruses using molecular beacons. Proceedings of the National Academy of Sciences, USA (1999) 96: 6394-6399.
Rys et al. Preventing False Positives: Quantitative Evaluation of Three Protocols for Inactivation of Polymerase Chain Reaction Amplification Products. Journal of Clinical Microbiology (1993) 31(9): 2356-2360.
Caparros-Wanderley et al. Intratype sequence variation among clinical isolates of the human papillomavirus type 6 L1 ORF: clustering of mutations and identification of a frequent amino acid sequence variant. Journal of General Virology (1999) 80: 1025-1033.
GenBank Accession No. AF067160.1 for the L1 gene of an HPV-6 deletion mutant, Apr. 13, 1999 [online], [retrieved on May 9, 2010], retrieved from the Internet: <URL: //www.ncbi.nlm.nih.gov
uccore/4039082>.
Tyagi et al. Molecular Beacons: Probes that Fluoresce upon Hybridization. Nature Biotechnology (1996) 14: 303-308.
Ramachandran et al. Target discrimination by surface-immobilized molecular beacons designed to detect Francisella tularensis. Biosensors and Bioelectronics (2004) 19: 727-736.
GenBank Accession No. AF335603.1 for the L1 gene of an HPV-11 isolate, Mar. 20, 2001 [online], [retreived on May 10, 2010], retrieved from the Internet: <URL: //www.ncbi.nlm.nih.gov
uccore/13384100>.
Szemes et al. Design of molecular beacons for AmpliDet RNA assay : Characterization of binding stability and probe specificity. Analytical Biochemistry (2003) 315: 189-201.
Coutlee et al., “Use of PGMY Primers in L1 Consensus PCR Improves Detection of Human Papillomavirus DNA in Genital Samples,” 2002, pp. 902-907, Journal of Clinical Microbiology, V. 40, No. 3.
Deng et al., “Two-dimensional micro-bubble actuator array to enhance the efficiency of molecular beacon based DNA micro-biosensors,” 2006, pp. 143-1450, Biosensors and Bioelectronics 21.
De Villiers, “Heterogeneity of the Human Papillomavirus Group,” 1989, pp. 4898-4903, Journal of Virology, V. 63, No. 11.
El Hajj et al., “Detection of Rifampin Resistance in Mycobacterium tuberculosis in a Single Tube with Molecular Beacons,” 2001, pp. 4131-4137, Journal of Clinical Microbiology, V. 39, No. 11.
Hsieh et al., “An efficient algorithm for minimal primer set selection,” 2003, pp. 285-286, Bioinformatics Applications Note, V. 19, No. 2.
Hubbard, “Human Papillomavirus Testing Methods,” 2003, pp. 940-945, Arch Pathol Lab Med, V. 127.
Kleter et al., “Development and Clinical Evaluation of a Highly Sensitive PCR-Reverse Hybridization Line Probe Assay for Detection and Identification of Anogenital Human Papillomavirus,” 1999, pp. 2508-2517, Journal of Clinical Microbiology, V. 37, No. 8.
Nilsson et al., “Real-time monitoring of rolling-circle amplification using a modified molecular beacon design,” 2002, pp. 1-7, Nucleic Acids Research, V. 30, No. 14.
Ramachandran et al., “Capillary electrophoresis and fluorescence studies on molecular beacon-based variable length oligonucleotide target discrimination,” 2003, pp. 70-77, Electrophoresis, V. 24.
Snijders et al., “The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes,” 1990, pp. 173-181, Journal of General Virology, V. 71.
Strohsahl et al., “Towards single-spot multianalyte molecular beacon biosensors,” 2005, pp. 479-485, Talanta, V. 67.
Szuhai et al., “A Novel Strategy for Human Papillomavirus Detection and Genotyping with SybrGreen and Molecular Beacon Polymerase Chain Reaction,” 2001, pp. 1651-1660, American Journal of Pathology, V. 159, No. 5.
Tsourkas et al., “Shedding light on health and disease using molecular beacons,” 2003, pp. 372-384, Briefings in Functional Genomics and Proteomics, V. 1, No. 4.
Tsourkas et al., “Structure-function relationships of shared-stem and conventional molecular beacons,” 2002, pp. 4208-4215, Nucleic Acids Research, V. 30, No. 19.
Tsourkas et al., “Hybridization kinetics and thermodynamics of molecular beacons,” 2003, pp. 1319-1330, Nucleic Acids Research, V. 31, No. 4.
Vogelstein et al., “Digital PCR,” 1999, pp. 9236-9241, Proc. Natl. Acad. Sci. USA, V. 96.
International Search Report from the International Searching Authority for International Application No. PCT/GB2006/004266, mailed Jun. 11, 2007, 13 pages.
Written Opinion of the International Searching Authority for International Application No. PCT/GB2006/004266, mailed Jun. 11, 2007, 15 pages.
European Search Report from the European Patent Office for European Application No. EP 06 80 8556.2, mailed Feb. 12, 2008, 5 pages.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for detecting pathogens using molecular beacons does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for detecting pathogens using molecular beacons, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting pathogens using molecular beacons will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2790795

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.