Method for detecting nucleic acids through a triple-stranded DNA

Details Method for detecting nucleic acids through a triple-stranded DNA Method for detecting nucleic acids through a triple-stranded DNA

1998-11-19

2000-10-17

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 19, 435 23, C12Q 168, C12Q 144, C12Q 137

Patent

active

06132972&

ABSTRACT:
A nucleic acid detecting method using a probe is provided which makes it possible to obtain correct information on a target double-stranded DNA dsDNA without damaging the target double-stranded DNA dsDNA. In the nucleic acid detecting method of the present invention, a nucleic acid is detected by subjecting the target double-stranded DNA dsDNA bound with a first single-stranded DNA dsDNA probe via recA protein to a treatment with a single-stranded DNA dsDNA specific nuclease, to thereby allow a second single-stranded DNA dsDNA probe consisting of a base sequence complementary to that of the first probe or a part thereof and labeled with a detectable marker to bind to the target DNA dsDNA.

REFERENCES:
patent: 5460941 (1995-10-01), Camerini-Otero et al.
patent: 5707811 (1998-01-01), Ferrin et al.
patent: 5750338 (1998-05-01), Collins et al.
Cheng, Suzanne et al., "Use of Psoralen-modified Oligonucleotides to Trap Three-stranded RecA-DNA Complexes and Repair of These Cross-linked Complexes by ABC Excinuclease"; The Journal of Biological Chemistry; 263(29):15110-15117; 1988.

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