Method for detecting nucleic acid amplification using self-quenc

Details Method for detecting nucleic acid amplification using self-quenc Method for detecting nucleic acid amplification using self-quenc

1994-11-16

1996-07-23

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 5, 435 912, 536 243, 536 2431, 536 2433, 536 266, C12Q 168, C12Q 170, C12P 1934, C07H 2104

Patent

active

055388485

ABSTRACT:
A method is provided for monitoring the progress of nucleic acid amplifications that rely on a nucleic acid polymerase having 5'.fwdarw.3' exonuclease activity. An important feature of the method is providing an oligonucleotide probe having a reporter molecule and a quencher molecule at either end such that the quencher molecule substantially quenches any fluorescence from the reporter whenever the oligonucleotide probe is in a single stranded state and such that the reporter is substantially unquenched whenever the oligonucleotide probe is in a double stranded state hybridized to a target polynucleotide.

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