Method for detecting mutation and a method for inducing...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S473000, C435S410000, C435S440000, C536S023100

Reexamination Certificate

active

07638298

ABSTRACT:
A novel plant transposon that induces bud mutation, transposase thereof, and a method for efficiently inducing bud mutation by improvement in the transposition efficiency are provided. A novel transposon having terminal inverted repeat sequences (SEQ ID NO: 1 and SEQ ID NO: 2) shown below has been isolated for the first time from carnation (Dianthus caryophyllus) and the nucleotide sequence thereof has been determined. The transposon has contained a gene encoding the transposase in its sequence. The transposon induces bud mutation when it is inserted into a gene. The transposition frequency is elevated due to environmental stress. TAGAGCTGGCAAA---TTTGCCAGCTCTA (SEQ ID NOS 1 & 2, respectively in order of appearance).

REFERENCES:
patent: 7132587 (2006-11-01), Kikuchi et al.
patent: 2003-093074 (2003-04-01), None
Guo et al, Protein tolerance to random amino acid change, PNAS, 2004, vol. 101 (25), pp. 9205-9210.
Lesk et al, Prediction of Protein Function from Protein Sequence and Structure, p. 27 and 28, downloaded Sep. 16, 2007.
D.R. Lisch et al., “Mutator transposase is widespread in the grasses”, Plant. Physiol., 2001, vol. 125, No. 3, pp. 1293-1303.
X. Zhang et al., “P instability factor: an active maize transposon system associated with the amplification of Tourist-like MITEs and a new superfamily of transposases”, Proc. Natl. Acad. Sci. USA, 2001, vol. 98, No. 22, pp. 12572-12577.

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