Method for detecting molecules containing amine or thiol groups

Details Method for detecting molecules containing amine or thiol groups Method for detecting molecules containing amine or thiol groups

1985-10-23

1987-07-14

Richman, Barry S.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436111, 436120, 436172, G01N 2177, G01N 3352, G01N 3368

Patent

active

046802720

ABSTRACT:
A stain for detecting molecules having amine or thiol groups, comprising halogenated maleimides and derivatives thereof, such as halogenated maleicdiamides. Upon binding to nitrogen- or sulfur-containing molecules, such as proteins, the halogenated stains undergo a chemical transformation which causes them to fluoresce. By applying the stain to a substrate suspected of containing amine or thiol groups and applying an ultraviolet light source thereto, any fluorescence, indicative of the presence of amines or thiols, can be readily detected.

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Oda et al., Tetrahedron, vol. 24, pp. 4051-4056, 1968.

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