Method for detecting microorganisms by separation and culture on

Details Method for detecting microorganisms by separation and culture on Method for detecting microorganisms by separation and culture on

1999-12-07

2000-08-22

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435975, 435 34, 435 4, 4352871, 4352881, C12Q 102, C12Q 104

Patent

active

06107053&

ABSTRACT:
A centrifuge tube and a kit for detecting the presence or absence of microorganisms, particularly bacteria and yeasts, in a liquid sample of a biological material. The centrifuge tube has a cylinder portion; a frustoconical portion; a capillary at the bottom of the tube; and contains a gel system for detecting microorganisms. The gel system includes a development phase of a gel containing microorganism culture medium and a reagent capable of inducing a detectable variation in optical measurement in the presence of microorganisms and a first phase intimate mixture which includes water originating wholly or partly from the culture medium and water-absorbing polymer particles so that, in the first phase intimate mixture, the polymer particles have a particular concentration and size.

REFERENCES:
patent: 3932222 (1976-01-01), Dorn
patent: 4131512 (1978-12-01), Dorn
patent: 4164449 (1979-08-01), Dorn et al.
patent: 4190328 (1980-02-01), Levine et al.
patent: 4212948 (1980-07-01), Dorn
patent: 4717660 (1988-01-01), Schulte
patent: 6020150 (2000-02-01), Contant-Pussard et al.
Dorn et al., "Blood Culture Technique Based on Centrifygation: Developmental Phase", Journal of Clinical Microbiology, Mar. 1976, vol. 3, No. 3, pp. 251-257.

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