Method for detecting low levels of microorganisms

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 732, 435 792, 435 29, 435174, 436518, G01N 33569

Patent

active

054159970

ABSTRACT:
The invention relates to methods for the detection of low numbers of a particular microorganism or microorganisms from a mixed population, the method comprising exposing the sample to a solid support to which antibodies specific for the organism(s) are adsorbed and either growing the bound organism(s) to a detectable level followed by immunoassay or releasing the organisms from the support followed by growth on a non-selective medium and observation of the resultant colonies. The invention also relates to test kits for performing these methods.

REFERENCES:
patent: 3970518 (1976-07-01), Giaever
patent: 4563418 (1986-07-01), Ward, Jr.
patent: 4592994 (1986-03-01), Mattiasson
patent: 4677055 (1987-06-01), Dodin
patent: 4683196 (1987-07-01), McLaughlin
Maggio, E. T., Ed., Enzyme Immunoassay, CRC Press, Inc. Boca Raton, 1980. pp. 167-179.
Rose et al., Manual of Clinical Laboratory Immunology, American Society for Microbiology, Washington, D.C., 1986. pp. 99-109.
Bailey et al., Diagnostic Microbiology. C. V. Mosby Co., St. Louis, 1974. pp. 377, 387, 193-194, 141, 144-147.
Macario, A. J. L., Ed., Monoclonal Antibodies against Bacteria, Academic Press, Orlando, 1985. p. xxii.
Mohit B et al; "A Simple Single-Step Immunoimmobilisation Method for the Detection of Salmonella in the Presence of Large Numbers of Other Bacteria" J Med Microbiol (1975) 8 173-176.
Bulletin OEPP/EEPO Bulletin, vol. 17, 11987, pp. 139-148, Wageningen, NL; J. W. L. Van Vuurde: "New Approach in Detecting Phytopathogenic Bacteria by Combined Immunoidentification Assays".
Van Vuurde et al. (1987) Principles and prospects of new serological techniques including immunosorbent immunofluorescence, immunoaffinity isolation and immunosorbent enrichment for sensitive detection of phytopathogenic bacteria, In Proceedings of the 6th International Conference on Plant Pathogenic Bacteria, Beltsvelle, 1985, Nijhofljunk. the Hague, pp. 835-842.
Hranitzky et al. (1980) Isolation of Ol serovars of Vibrio cholerae from water by serologically specific method. Science 210, 1025-1026.
Van Vuurde et al., (1983), Immunosorbent immunofluorescence microscopy (ISIF) and immunosorbent dilution plating (ISPD); new methods for the detection of plant pathogenic bacteria. Seed Science. & Technol. II, 523-533.
Van Vuurde et al., (1987), Detecting seedborne bacteria by immunofluorescence. In Proceedings of the 6th International Conference on Plant Pathogenic Bacteria, Beltsvelle, 1985. Nijhofljun. The Hague.
Farber, Jeffrey M. and Speirs, Joan L., "Monoclonal Antibodies Directed Against the Flagellar Antigens of Listeria Species and Their Potential in EIA-Based Methods", Journal of Food Protection, vol. 50, No. 6, pp. 479-484 (Jun. 1987).
Seeliger, H. and Hohne, K., "Serotyping of Listeria monocytogenes and Related Species", Methods in Microbiology, vol. 13, pp. 31-49, (1979).
Paterson, J., Stuart, "Flagellar Antigens of Organisms of the Genus Listerella", Institute of Animal Pathology, University of Cambridge, pp. 25-32.
Marfleet, Jane, E., and Blood, Ruth, M., "Listeria Monocytogenes as a Food-Borne Pathogen," Scientific and Technical Surveys, No. 157, Feb., 1987.
Lovett, Joseph, "Listeria Isolation", Bacteriological Analytical Manual, 6th edition, 1984, chapter 29.
La Roche et al; "Field Evaluation of the Membrane Filter-Disc Immunoimmobilization Technique in the Detection of Salmonellae in Egg Products" Poultry Science (1981) 60 2265-2269.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for detecting low levels of microorganisms does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for detecting low levels of microorganisms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting low levels of microorganisms will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-637166

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.