Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
2007-01-23
2007-01-23
Campell, Bruce H. (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C435S004000, C435S007100, C435S007200, C435S007320, C435S007500, C424S093100, C424S093600, C424S093300
Reexamination Certificate
active
10249452
ABSTRACT:
The invention is directed to a method for detecting low concentrations of bacteria in liquid solution that may or may not be complex liquid solutions. In one embodiment, immunomagnetic separation (IMS) is used to separate target bacterium that may be in a liquid mixture from other constituents in the mixture. A low concentration of a bacteriophage for the target bacteria is subsequently used to infect target bacterial cells that have been captured using the IMS technique. If at least a certain concentration of target bacterium are present, the bacteriophage will multiply to a point that is detectable. Matrix assisted laser desorption ionization/time-of-flight-mass spectrometry (MALDI/TOF-MS) is then used to produce a mass spectrum that is analyzed to determine if one or more proteins associated with the bacteriophage are present, thereby indirectly indicating that target bacterium were present in the liquid mixture.
REFERENCES:
patent: 4104126 (1978-08-01), Young
patent: 4797363 (1989-01-01), Teodorescu et al.
patent: 4861709 (1989-08-01), Ulitzur et al.
patent: 5085982 (1992-02-01), Keith
patent: 5168037 (1992-12-01), Entis et al.
patent: 5498525 (1996-03-01), Rees et al.
patent: 5620845 (1997-04-01), Gould et al.
patent: 5656424 (1997-08-01), Jurgensen et al.
patent: 5679510 (1997-10-01), Ray et al.
patent: 5723330 (1998-03-01), Rees et al.
patent: 5824468 (1998-10-01), Scherer et al.
patent: 5888725 (1999-03-01), Sanders
patent: 5914240 (1999-06-01), Sanders
patent: 5958675 (1999-09-01), Wicks et al.
patent: 5985596 (1999-11-01), Wilson
patent: 6037118 (2000-03-01), Thomas et al.
patent: 6090541 (2000-07-01), Wicks et al.
patent: 6265169 (2001-07-01), Cortese et al.
patent: 6300061 (2001-10-01), Jacobs, Jr. et al.
patent: 6355445 (2002-03-01), Cherwonogrodzky et al.
patent: 6428976 (2002-08-01), Chang et al.
patent: 6436652 (2002-08-01), Cherwonogrodzky et al.
patent: 6436661 (2002-08-01), Adams et al.
patent: 6461833 (2002-10-01), Wilson
patent: 6524809 (2003-02-01), Wilson
patent: 6544729 (2003-04-01), Sayler et al.
patent: 6555312 (2003-04-01), Nakayama
patent: 6660437 (2003-12-01), Friedrich et al.
patent: 6660470 (2003-12-01), Sanders
patent: 2002/0045195 (2002-04-01), Hubscher et al.
patent: 2002/0127547 (2002-09-01), Miller
patent: 2002/0192676 (2002-12-01), Madonna et al.
patent: 2003/0027241 (2003-02-01), Sayler et al.
patent: 2004/0137430 (2004-07-01), Anderson et al.
patent: 43 14998 (1994-11-01), None
patent: 0228975 (1987-07-01), None
patent: 0439354 (1991-07-01), None
patent: 1300082 (2003-04-01), None
patent: WO-93/17129 (1993-09-01), None
patent: WO-94/06931 (1994-03-01), None
patent: WO-95/05483 (1995-02-01), None
patent: WO-98/18962 (1998-05-01), None
patent: WO-02/061117 (2002-08-01), None
patent: WO-03/087772 (2003-10-01), None
patent: WO-03/087772 (2003-10-01), None
Chatterjee et al., FEMS Microbiology Letters, vol. 188 No. 1, pp. 47-53 (Jul. 2000).
Olsvik et al., Clinical Microbiology Reviews, vol. 7 No. 1, pp. 43-54 (Jan. 1994).
Sun et al., Journal of Industrial Microbiology & Biotechnology, vol. 27 No. 2, pp. 126-128 (Aug. 2001).
Nakamura et al., Biophysical and Biophysical Research Communications, vol. 289 No. 1, pp. 252-256 (Nov. 2001).
R. Bordner and J. Winter,Microbiological Methods for Monitoring the Environment: Water and Wastes, Dec. 1978, pp. i-xvi, 1-338, Environmental Monitoring and Support Laboratory, USEPA, Cincinnati, USA.
D.T. Kingsbury and S. Falkow (eds),Rapid Detection and Identification of Infectious Agents, papers given at an International Symposium held in Berkeley, CA, 1985, pp. i-xii, 1-296, Academic Press, Inc., Orlando, USA.
G.M. Wyatt, M.R.A. Morgan and H.A. Lee,Immunoassays for Food-Poisoning Bacteria and Bacterial Toxins, Nov. 1992, pp. i-xiii, 1-129, James & James and Chapman Hall, Windsor, London, England.
H. Yu and J. Bruno, “Immunomagnetic-Electrochemiliuminescent Detection ofEscherichia coliO157 andSalmonella Typhimuriumin Foods and Environmental Water Samples”,Applied and Environmental Microbiology, Feb. 1996, pp. 587-592, 62-#2, American Society for Microbiology, Washington, DC, USA.
J.M. Van Der Wolf, L.J. Hyman, D.A.C. Jones, C. Grevesse, J.R.C.M. Van Beckhoven, J.W.L. Vuurde and M.C.M. Perombelon, “Immunomagnetic Separation ofErwinia CaratovoraSubsp.Astrosepticafrom Potato Peel Extracts to Improve Detection Sensitivity on a Crystal Violet Pectate Medium or by PCR”,Journal of Applied Bacteriology, May 1996, pp. 487-495, 80-#5, Blackwell Science, United Kingdom.
D. Dziadkowiec, L.P. Mansfield and S.J. Forsythe, “The Detection ofSalmonellain Skimmed Milk Powder Enrichments Using Conventional Methods and Immunomagnetic Separation”,Letters in Applied Microbiology, 1995, pp. 361-364, 20, The Society for Applied Bacteriology—published by Blackwell Science, United Kingdom.
I.R. Grant, H.J. Ball and M.T. Rowe, “Isolation ofMycobacterium Paratuberculosisfrom Milk by Immunomagnetic Separation”,Applied and Environmental Microbiology, Sep. 1998, pp. 3153-3158, 64-#9, American Society for Microbiology, Washington, DC, USA.
K.S. Cudjoe, T. Hagtvedt and R. Dainty, “Immunomagnetic Separation ofSalmonellafrom Foods and Their Detection Using Immunomagnetic Particle (IMP)-ELISA”,International Journal of Food Microbiology, Sep. 1995, pp. 11-25, 27-#1, Elsevier Science, Amsterdam, The Netherlands.
E. Skjerve, L.M. Rorvik and O. Olsvik, “Detection ofListeria Monocytogenesin Foods by Immunomagnetic Separation”,Applied and Environmental Microbiology, Nov. 1990, pp. 3478-3481, 56-#11, American Society for Microbiology, Washington, DC, USA.
B.H. Pyle, S.C. Broadaway and G.A. McFeters, “Sensitive Detection ofEscherichia ColiO157:H7 in Food and Water by Immunomagnetic Separation and Solid-Phase Laser Cytometry”,Applied and Environmental Microbiology, May 1999, pp. 1966-1972, 65-#5, American Society for Microbiology, Washington, DC, USA.
A.J. Madonna, F. Basile, E. Furlong and K.J. Voorhees, “Detection of Bacteria from Biological Mixtures Using Immunomagnetic Separation Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry”,Rapid Communications in Mass Spectrometry, 2001, pp. 1068-1074, 15-#13, John Wiley & Sons, Ltd., Hoboken, USA.
M.S. Dubow, “Bacterial Identification—Use of Phages”, section inEncyclopedia of Virology 2e, (R.G. Webster and A. Granoff (eds.)), 1999, Academic Press, San Diego, USA.
D.W. Hirsch and L.D. Martin, “Rapid Detection ofSalmonellaspp. by Using Felix-O1 Bacterio-phage and High-Performance Liquid Chromatography”,Applied and Environmental Microbiology, Jan. 1983, pp. 260-264, 45-#1, American Society for Microbiology, Washington, DC, USA.
S.J. Pugh, J.L. Griffiths, M.L. Arnott and C.S. Gutteridge, “A Complete Protocol Using Conductance for Rapid Detection of Salmonellas in Confectionary Materials”,Letters in Applied Microbiology, 1988, pp. 23-27, 7, The Society for Applied Microbiology—published by Blackwell Science, United Kingdom.
G.S.A.B. Stewart, S.A.A. Jassim, S.P. Denyer, P. Newby, K. Linley and V.K. Dhir, “The Specific and Sensitive Detection of Bacterial Pathogens With 4h Using Bacteriophage Amplification”,Journal of Applied Microbiology, 1988, pp. 777-783, 84, The Society for Applied Microbiology—published by Blackwell Science, United Kingdom.
G.S.A.B. Stewart, “A ReviewIn VivoBioluminescence: New Potentials for Microbiology”,Letters in Applied Microbiology, 1990, pp. 1-8, 10, The Society for Applied Microbiology—published by Blackwell Science, United Kingdom.
C.P. Kodikara, H.H. Crew and G.S.A.B. Stewart, “Near On-Line Detection of Enteric Bacteria Using Lux Recombinant Bacteriophage”,FEMS Microbiology Letter, 1991, pp. 261-265, 83, Federation of European Microbiological Societies—published by Elsevier, The Netherlands.
S.J. Favrin, S.A. Jassim and M.W. Griffiths, “Development and Optimizatin of a Novel Immunomagnetic S
Madonna Angelo J.
Rees Jon C.
Voorhees Kent J.
Campell Bruce H.
Colorado School of Mines
Holland & Hart LLP
Kulish, Esq. Christopher J.
Lucas Zachariah
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