Method for detecting Listeria monocytogenes via esculin hydrolys

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 681, C12Q 168, C12P 2106

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active

060015594

ABSTRACT:
A method for rapidly detecting non-viral organisms disclosed. The method involves pelleting an enrichment culture of a sample, optionally washing the pellet, resuspending the pellet in an appropriate reagent before performing a nucleic acid hybridization confirmation test on the sample.

REFERENCES:
patent: 5089386 (1992-02-01), Stackebrandth et al.
Fraser & Sperber, "Rapid Detection of Listeria spp. in Food & Environmental Samples by Esculin Hydrolysis," Journal of Food Protection, vol. 51, #10, pp. 762-765 (1988).
Okumabula, et al. "Evaluation of a Chemiluminescent DNA Assay for the Rapid Detection of Listeria Monocytogenes," Research Microbiology, vol. 143, pp. 183-189 (1992).
Accu-Probe "Listeria Monocytogenes Culture Identification Test" Protocol (1990).
J. Cin. Microbiol 4(2)[1976]: 180-184.
Edberg, S.C; et al "Rapid Spot Test for the Determination of Esculin Hydrolysis" Proc. Natl. Acad. Sci. U.SA 79:6999-7003 (1982).
Wirth D.F; et al Rapid Identification of Leishmania Species by Specific Hydridization of Kinetoplast DNA in Cutaneous Lesions.

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