Method for detecting intestinal pathogen dientamoeba fragilis

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 29, 436 63, 436164, 436800, 424 3, 424 71, C12Q 104, G01N 3348

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active

053345096

ABSTRACT:
A method and apparatus for producing detectable intestinal parasites. The method includes obtaining an intestinal mucosa sample (e.g. feces) having intestinal parasites, such as Dienamoeba fragilis; and contacting the obtained intestinal mucosa sample with an acridine base compound (e.g. acridine orange and/or acridine yellow, etc.) such that the intestinal parasites become differentially stained and detectable by a human eye when viewed through a fluorescence microscope. The apparatus includes a kit or the like which includes at least one vessel or vial. Preferably, two vials are contained within the kit with one vial having an isotonic salt solution including a salt, such as sodium chloride, potassium phosphate, etc., and the other vial containing an acridine biological staining compound.

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