Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-10-30
1991-09-10
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436518, 436514, 436533, 436548, 435 724, 435 792, 435 793, 435 74, G01N 3353, G01N 33543
Patent
active
050473239
ABSTRACT:
Two novel cell lines, ATCC #HB-8963 and ATCC #HB-8964 produce monoclonal antibody to human kininogen. One of the antibodies specifically recognizes the heavy chain of high and low molecular weight kininogen (the later protein is identical to alpha cysteine protease inhibitor). The other antibody recognizes the light chain of high molecular weight kininogen. The hybridomas are formed by fusing spleen cells from immunized BALB/c AnSkh mice with P3X63Ag8 or SP2/0-Ag14 myeloma cells. Diagnostic, therapeutic and biochemical uses of the monoclonal antibodies are provided.
REFERENCES:
Bedi, G. S. et al., "Single Step Purification of `Kallikrein-Resistant` Kininogen from Rat Plasma Using Monoclonal-Antibody Immunoaffinity Chromatography", Preparative Biochemistry, 15(3): 159-169, 1985.
Schmaier, A. H. et al., "High-Molecular Weight Kininogen: A Secreted Platelet Protein", J. Clin. Invest., 71: 1477-1489, 1983.
Goding, J. W., Monoclonal Antibodies: Principles and Practice, Academic Press, Inc., Orlando, 1983, pp. 56-97.
Berrettini, M. et al., "Immunological Studies of Human High Molecular Weight Kininogen (HMWK) Using Murine Monoclonal Antibodies (Mab)", Thrombosis and Hemostasis, 54(1): 229, Jul. 14, 1985.
Muller-Esterl et al., "Purification and Properties of Human Low Molecular Weight Kininogen", Biochimica et Biophysica Acta, 706, 145-152 (1982).
Kerbiriou et al., J. Biol. Chem., 255, 3952-3958 (1980).
Colman Robert W.
Schmaier Alvin H.
Kepplinger Esther L.
Scheiner Laurie A.
Temple University Of The Commonwealth System Of Higher Education
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