Method for detecting hepatitis C virus

Details Method for detecting hepatitis C virus Method for detecting hepatitis C virus

2005-07-05

2005-07-05

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200, C536S023100, C536S024300, C536S025300, C536S026600

Reexamination Certificate

active

06913887

ABSTRACT:
A method for detecting hepatitis C virus (HCV) in a sample includes the steps of (a) extracting RNA from the sample; (b) contacting the RNA to a reverse transcriptase and a downstream primer specific to the HCV RNA sequence to create cDNA; (c) forming an amplification medium by mixing the cDNA with a PCR reaction mixture, a nucleic-acid-binding fluorescent entity, and the downstream primer and an upstream primer specific to the HCV RNA sequence; (d) thermally cycling the amplification medium between at least a denaturation temperature and an elongation temperature; (e) illuminating the amplification medium with a selected wavelength of light that is absorbed by the fluorescent entity during the thermally cycling step; (f) determining the amount of fluorescence generated by the fluorescent entity; and (g) detecting the presence of the target nucleic acid by analyzing the amount of luminescence determined after at least one amplification cycle.

REFERENCES:
patent: 2003/0124512 (2003-07-01), Stuyver
patent: 2004/0024193 (2004-02-01), Williams

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