Method for detecting hepatitis associated antigen

Chemistry: physical processes – Physical processes – Crystallization

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424 8, 424 12, 424 89, 424287, G01N 3102, G01N 3316

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active

040134113

ABSTRACT:
A method for testing biological materials, and especially those obtained from the human body, for the presence of the hepatitis associated antigen (HAA), in which Zeise's salt, or another water soluble platinum or palladium salt containing a trans directing group which enables the noble metal-containing moiety of the salt to react with the hepatitis associated antigen (HAA) or related materials, is mixed with the material to be tested in the presence of water, causing the formation of characteristic visible turbidity in the mixture which persists after agitation if HAA is present. Any slight turbidity observed initially in normal sera disappears upon agitation if the test sample is free from the hepatitis associated antigen. Also disclosed are test reagents consisting of the platinum and palladium salts in aqueous solutions containing non-interfering stabilizing salts such as sodium and potassium chloride and equivalents.

REFERENCES:
blumberg, Nat. Acad. Med., 44:112, 1566 (1968).
Alter, Blood, 27:3, 297 (1966).
Zuckerman, Nature, 223:569 (1969).
Anderson; J. Chem. Soc. (London) pt. 2, 1936 pp. 1042-1049.
Keller, Chem. Rev., vol. 28, 1941 pp. 229-234, 239-244.

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