Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2003-04-28
2008-10-07
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C435S041000, C435S028000
Reexamination Certificate
active
07432073
ABSTRACT:
The present invention relates to a method capable of detecting proliferative bacteria in food and beverage, particularly acid or neutral beverage at low cost within a short time, a detection kit which enables simple measurement, and a technology for examination of harmful bacteria in food and beverage with respect to a method for rapid determination of proliferation properties of bacteria capable of producing guaiacol in food and beverage.There is disclosed a method for detecting bacteria capable of producing guaiacol, which comprises culturing or incubating a culture solution containing a specimen for a predetermined time in the presence of vanillic acid, and measuring the produced guaiacol by qualitative or quantitative analysis.Also there is disclosed a kit for detection of bacteria capable of producing guaiacol, comprising a container, and an acid or neutral culture medium containing a vanillic acid substrate for production of guaiacol, which is contained in the container and also sterilized and sealed.Furthermore there is disclosed a method for rapid determination of proliferation properties of bacteria capable of producing guaiacol in food and beverage, which comprises incubating a sporophyte of spore-forming bacteria capable of producing guaiacol for a predetermined time in the food and beverage in the presence of vanillic acid, and measuring the produced guaiacol by qualitative or quantitative analysis.
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Patent Abstracts of Japan for JP7-68 published on Jan. 6, 1995.
Kawamoto Atsuko
Kuriyama Akemi
Niwa Motohiro
Darby & Darby P.C.
Gitomer Ralph
Kirin Beverage Corporation
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