Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-04-28
1997-01-28
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435794, 435967, 435969, 435970, 436518, 436530, 436810, 422 56, 422 58, 422 60, 53038824, G01N 3353
Patent
active
055977006
ABSTRACT:
A method and device for detecting fetal membrane rupture by detecting only that insulin-like growth-factor-binding protein 1 (IGFBP-1) which is free of insulin-like growth factors 1 and 2 (IGF-1 and IGF-2) are disclosed. The method is based on the fact that the concentration of free IGFBP-1 in the blood serum is about 10 times lower than that of the IGFBP-1 bound to IGF-1 and IGF-2, while in the amniotic liquid which is contained in the amnion has more than 1000 times the concentration of free IGFBP-1 than the blood serum. Therefore, when even a small amount of amniotic liquid is contained in the vaginal secretion sample, this is clear evidence of the rupture of the fetal membrane having taken place. The method and the device make it possible to diagnose fetal membrane rupture with an extremely high accuracy. Because of the low concentration of the IGFBP-1 in the blood, the admixture of the blood serum to the sample does not affect the results of the test. The device comprises a one-pad-two-strips test kit which may be used to detect free IGFBP-1 in a range of concentrations from 5 ng/ml to 250 .mu.g/ml.
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Boltovskaya Marina
Foux Boris
Konstantinov Alexander
Nazimova Svetlana
Starosvetskaya Nelli
California Research LLC
Dehlinger Peter J.
Evans Susan T.
Pressman David
Spiegel Carol A.
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