Method for detecting Escherichia coli

Details Method for detecting Escherichia coli Method for detecting Escherichia coli

2006-05-09

2006-05-09

Sitton, Jehanne (Department: 1634)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S024300, C536S024320, C536S024330

Reexamination Certificate

active

07041811

ABSTRACT:
Specific nucleic acid sequences, e.g., SEQ ID NOs: 1–8, for detectingEscherichia coli. Also disclosed is a method of detectingEscherichia coli. The method includes providing a sample having a nucleic acid from an unknown microorganism; amplifying the nucleic acid with an upstream primer containing SEQ ID NO: 1 or 3 and a downstream primer containing SEQ ID NO: 2 or 4, each primer being 18–40 nucleotides in length; and detecting an amplification product. Detection of the amplification product, e.g., using SEQ ID NO: 5, 6, 7, or 8 as a probe, indicates the presence ofEscherichia Coli.

REFERENCES:
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4816389 (1989-03-01), Sansonetti et al.
patent: 5084565 (1992-01-01), Parodos et al.
patent: 5354661 (1994-10-01), Doyle et al.
patent: 5374718 (1994-12-01), Hammond et al.
patent: 5693469 (1997-12-01), Hogan
patent: 6060252 (2000-05-01), Hellyer et al.
patent: 6207818 (2001-03-01), Hellyer et al.
patent: WO 93/03186 (1993-02-01), None
Genbank Accession No. AE005490 (first appeared in Genbank on Jan. 25, 2003).
Genbank Accession No. AE00346 (Dec. 1, 2000).
Genbank accession No. Z70523 (Apr. 1996).
Genbank accession No. D90887 (1997).
Buck et al; Biotechniques, vol. 27, pp. 528-536, 1999.
Tijhie et al; J. Microbiol. Meth. vol. 18, pp. 137-150, 1993.
Accession No. AX002476 (Mar. 2000).
Accession No. AF175847 (Nov. 2000).
Genbank accession No. AE015280; Oct. 2002.
Genbank accession No. AE015281; Oct. 2002.
Allignment for Genbank Nos. and SEQ ID Nos. 1-8.
Mansfield et al; The Journal of Infectious Diseases, vol. 184, pp. 803-807, 2001.
Oswald et al (Infection and Immunity, vol. 68, pp. 64-71; 2000.

Affiliated with

Also associated with

Rating

Method for detecting Escherichia coli does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for detecting Escherichia coli, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting Escherichia coli will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3620711