Method for detecting elevated activity of oxidases in insects

Details Method for detecting elevated activity of oxidases in insects Method for detecting elevated activity of oxidases in insects

2007-05-29

2007-05-29

Gitomer, Ralph (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

C435S029000, C435S348000

Reexamination Certificate

active

10321723

ABSTRACT:
A method for checking for elevated activity of mixed-function oxidases using a single insect, includes homogenizing a single insect in an acid pH buffer, preferably by grinding it in a small tube, such as an Eppendorf tube, with a pestle, adding a buffered tetramethylbenzidine solution as substrate, adding hydrogen peroxide as oxidant, and checking the blue coloration, the intensity of which indicates the degree of elevated mixed function oxidase activity. The invention also provides a test kit for use in the method which comprises separate containers of: I. an acid pH buffer, preferably an acid buffer about pH5 II. tetramethylbenzidine III. a solvent for tetramethylbenzidine, preferably methanol, and IV. hydrogen peroxide.

REFERENCES:
patent: 5871939 (1999-02-01), Trowell et al.
patent: 2003/0100025 (2003-05-01), O'Connor et al.
patent: 2004/0101925 (2004-05-01), Lee et al.
Vulule et al, Medical & Vetinary Entomology, v. 13(3), p. 239, Sep. 1999, (Abstract Only).

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