Method for detecting coliform organisms

Details Method for detecting coliform organisms Method for detecting coliform organisms

1981-10-26

1983-05-31

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 3, 435 34, 435 38, 435807, C12Q 106, C12Q 300, C12Q 104, C12Q 110

Patent

active

043861571

ABSTRACT:
A method and apparatus are disclosed for determining the concentration of coliform bacteria in a sample. The sample containing the coliform bacteria is cultured in a liquid growth medium. The cultured bacteria produce hydrogen and the hydrogen is vented to a second cell containing a buffer solution in which the hydrogen dissolves. By measuring the potential change in the buffer solution caused by the hydrogen, as a function of time, the initial concentration of bacteria in the sample is determined. Alternatively, the potential change in the buffer solution can be compared with the potential change in the liquid growth medium to verify that the potential change in the liquid growth medium is produced primarily by the hydrogen gas produced by the coliform bacteria.

REFERENCES:
patent: 3740320 (1973-06-01), Arthur
patent: 3907646 (1975-09-01), Wilkins et al.
patent: 4009078 (1977-02-01), Wilkins et al.
patent: 4030979 (1977-06-01), Zuber
patent: 4204037 (1980-05-01), Dill et al.
patent: 4209299 (1980-06-01), Carlson
patent: 4246343 (1981-01-01), Wilkins et al.

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