Method for detecting charged oligonucleotides in biological flui

Details Method for detecting charged oligonucleotides in biological flui Method for detecting charged oligonucleotides in biological flui

1993-11-16

1996-04-09

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 792, 536 254, C12Q 168, G01N 3353, C07H 2100

Patent

active

055061036

ABSTRACT:
Disclosed is a method for detecting and quantitating oligonucleotides with charged internucleotide linkages in biological fluids. In this method, a biological fluid sample is contacted with an anion exchange resin at from 40.degree. C. to 65.degree. C. for a time sufficient to enable oligonucleotides in the sample to adsorb to the resin. The absorbed oligonucleotides are then desorbed with a buffer having a salt concentration of about 1 M to 2.5 M and a pH in the range of about 6.5 to 7.5, the desorption being performed at about 40.degree.-65.degree. C. The oligonucleotides so released are then detected and quantitated.

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