Method for detecting characteristic markers of disease in biolog

Chemistry: analytical and immunological testing – Optical result

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424 3, 424 71, 436 63, 436 64, 436 86, 436 94, 436169, G01N 2177

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050396196

ABSTRACT:
A method for detecting characteristic markers of disease in biological fluids including the steps of applying the biological fluid containing the marker onto a support matrix; differential staining with a staining mixture, selective for the marker and directly detecting the characteristic marker of disease.

REFERENCES:
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Orford et al., "Ultrastructural Alterations in Glycosaminoglycans of Dog Femoral Condylar Cartilage After Surgical Division of an Anterior Cruciate Ligament: a Study with Cupromeronic Blue in a Critical Electrolyte Concentration Technique", Journal of Anatomy, vol. 148, pp. 233-244, 1986.
"Glycosaminoglycans in Human Gingival Crevicular Fluid as Indicators of Active Periodontal Disease," K. S. Last, J. B. Stanbury and G. Embery, Archives of Oral Biology, vol. 30, No. 3, pp. 275-281 (1985).
"Characterization of Glycosaminogylcan-Alcian Blue Complexes by Elution from Cellulose Acetate Utilizing Different MgCl.sub.2 Concentrations," L. Hronowski and T. Anastassiades, Analytical Biochemistry, vol. 107, pp. 393-405 (1980).

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