Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1995-06-06
1997-07-15
Campbell, Eggerton A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
536 237, C07H 2104, C07K 1400
Patent
active
056484666
ABSTRACT:
The invention is directed to a method of detecting infection of a blood sample by various strains of bovine diarrhoea virus (BVD) including a first test wherein anti-bovine diarrhoea virus antibodies are detected by means of a recombinant antigen comprising the BVD p80 protein expressed by a eukaryotic host and a second test wherein viral particles are detected by means of antibodies directed against the BVD p80 viral protein. The method is particularly useful in detecting pathogenic conditions such as persistent viremias and acute infections caused by BVD viruses, and is particularly advantageous because it is an extremely sensitive and efficient test and because it is capable of detecting infections caused by any one of a wide variety of BVD strains. The recombinant antigen employed in the method is preferably encoded by the nucleotide sequence listed as SEQ ID No: 1.
REFERENCES:
Collett et al, Virology 165:191-199 (1988).
Collett, et al, Virology 165:200-208 (1988).
Kwang et al, Biochem. Biophy. Res. Comm 178:1326-1334 (1991).
Murhammer, App. Biochem. Biotech. 31:283-292 (1991).
Chappuis Gilles-Emile
LeComte Corine Martine Therese Ghislaine
Pin Jean-Jacques
Vandenbergh Danielle Marie Helene Jeanne
Campbell Eggerton A.
Rhone Merieux
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