Method for detecting bacterial spores

Chemistry: molecular biology and microbiology – Spore forming or isolating process

Reexamination Certificate

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C424S001110, C424S001170, C435S004000, C435S007200, C435S007320, C435S030000, C435S034000, C435S243000, C435S244000, C435S252100, C435S252310, C436S161000, C436S173000, C436S174000, C436S177000

Reexamination Certificate

active

07547536

ABSTRACT:
A method of detecting the presence and quantity of bacterial spores, which includes adding an electrophilic alcohol and an acid anhydride to a sample, admixing the sample with a solvent, and analyzing the sample. The sample may be analyzed by injecting the mixture into a gas chromatograph equipped with a mass spectra detector.

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Beverly et al. 2000. Anal. Chem. vol. 72: 2428-2432.
Beverly et al. 1996. Rapid Comm. in Mass Spectro. vol. 10:455-458.
Synder et al. 1996. Field Anal. Chem. and Tech. vol. 1(1):49-58.
Beverly et al., “Analysis of Dipicolonic acid in Bacterial Spores by Electron Monochromator-Mass Spectrometry,”47thASMS Conference on Mass Spectrometry and Allied Topics, Dallas, Texas, Jun. 1999.
Beverly et al., “Electron Monochromator Mass Spectrometry for the Analysis of Whole Bacteria and Bacterial Spores,”Analytical Chemistry, vol. 72, No. 11, pp. 2428-2432, Jun. 2000.

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