Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1998-03-19
1999-10-19
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 34, 435 38, C12Q 134
Patent
active
059687622
ABSTRACT:
The invention is a method for detecting the presence of bacteria in a sample. The method of the invention utilizes the steps of (a) admixing a fluorescent conjugate with a sample to be tested for the presence of bacteria in the sample, wherein a moiety of the fluorescent conjugate is metabolized to release the fluorescent moiety of the fluorescent conjugate; (b) inducing fluorescence of the fluorescent conjugate and the fluorescent moiety of the metabolized fluorescent conjugate at 325-345 nm; (c) simultaneously detecting the fluorescence of the fluorescent conjugate at 370-380 nm and the fluorescent moiety of the metabolized fluorescent conjugate at 445-480 nm; and (d) correlating the detected fluorescence of the fluorescent conjugate and the fluorescent moiety of the metabolized fluorescent conjugate to the presence of bacteria in the sample.
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Anderson Carol Patricia
Bauman Robert
Jadamec Joseph Richard
Jakubielski Stephen A.
Kovacs Michael J.
Garabedian Todd E.
Gitomer Ralph
Simons William A.
The University Of Connecticut
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