Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1994-05-27
1997-01-07
Kepplinger, Esther
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, C12Q 118
Patent
active
055915990
ABSTRACT:
A method for determining the possible presence of an antimicrobial compound in a sample. The method includes the steps of: (i) adding the sample to a culture medium; (ii) growing a microorganism in the culture medium, the growth of the microorganism being susceptible to inhibition by the compound; (iii) measuring an electrical parameter of the culture medium over a period of time; (iv) determining a first time point at which an accelerating change of the electrical parameter occurs, the change resulting from the growth of the microorganism; and (v) comparing the first time point with a plurality of time points to determine the possible presence of the compound. Each of the plurality of time points is determined in a manner identical to that in which the first time point is determined except that the compound is present in the culture medium at a known concentration, and, further, a relationship exists between the plurality of time points and their corresponding concentrations. Also disclosed is a method for testing the resistance of a microorganism to an antimicrobial compound.
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Okigbo et al., "Detection of Penicillin and Streptomycin in Milk by Impedance Microbiology", Journal of Food Protection, vol. 48 No. 11, pp. 979-981, Nov. 1985.
Chang Tsung C.
Chen Hui C.
Food Industry Research and Development Institute
Kepplinger Esther
Tran Lien
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