Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-04-24
1999-06-01
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 8, 435 29, 435 39, 435259, 435267, 435268, 435968, 435975, C12Q 168, C12Q 166, C12Q 102, G01N 3353
Patent
active
059087510
ABSTRACT:
The present invention provides a method for detecting and/or determining the ATP from microorganism cells in a sample, which comprises the steps of: centrifuging the sample and removing the supernatant, thereby forming a microorganism cell pellet; adding to the microorganism cell pellet a buffer containing a protease-free soluble protein and an ATP hydrolase and incubating the mixture at a pH of 6.0-8.0; extracting ATP from the microorganism cells with an added ATP extraction agent; and detecting and/or determining the ATP released from the microorganism cells by bioluminescence analysis. The present invention also provides a test kit for detecting and/or determining the ATP from microorganism cells, which comprises a reagent containing a buffer capable of pH adjustment to 6.0-8.0, a protease-free soluble protein and an ATP hydrolase, a reagent containing an ATP extraction agent, and a bioluminescence reagent.
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patent: 4668623 (1987-05-01), Kinnunen et al.
patent: 5258285 (1993-11-01), Aegidius
patent: 5700645 (1997-12-01), Pahuski et al.
Higo Sachiko
Tanaka Reiko
Lankford , Jr. Leon B.
Tate Christopher R.
Toyo Ink Mfg. Co. Ltd.
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