Chemistry: analytical and immunological testing – Tracers or tags
Patent
1993-09-22
1996-07-30
Warden, Jill
Chemistry: analytical and immunological testing
Tracers or tags
436164, 436172, 422 52, 422 8505, 422 8206, 422 8208, G01N 2100
Patent
active
055411134
ABSTRACT:
A method for detecting an analyte, in an aqueous solution at a physiological pH, by reductive or oxidative/reductive electrochemical luminescence methodologies is disclosed. The method proceeds by labelling the analyte with a transition metal complex, followed by inducing the transition metal label to luminescence by application of a suitable electrical potential to a solution containing the label and the analyte. The transition metal complex can be a tris-ruthenium(bipyridine) complex. A hydroxylamine and/or a halogen-containing moiety can be used to enhance both reductive and/or oxidative electrochemical luminescence of the transition metal complex. The transition metal chelate can be used as a label for the detection of picomolar concentrations of an analyte of interest, such as an analyte present in a sample of a physiological fluid.
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Siddigi Iqbal W.
Sternberg James C.
Beckman Instruments Inc.
Carrillo Sharidan
Henry Janis C.
May William H.
Warden Jill
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