Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-04-05
1999-02-23
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 935 77, 935 78, C12Q 168, C12P 1934
Patent
active
058742182
ABSTRACT:
This invention discloses novel detection methods for determining the presence of a target compound in a substance using nucleic acid ligands as detection molecules. Specifically, the substance is bound to a solid support matrix, such as those used in blot procedures, and detection of the target molecule is accomplished using the affinity and specificity of nucleic acid ligands to the target molecule. The method utilized herein for identifying and preparing said nucleic acid ligands is called SELEX. The method of the present invention is additionally useful to isolate the target compounds from the various substances.
REFERENCES:
patent: 5270162 (1993-12-01), Gold et al.
patent: 5270170 (1993-12-01), Schatz et al.
patent: 5359047 (1994-10-01), Donahue et al.
patent: 5436144 (1995-07-01), Stewart et al.
patent: 5470878 (1995-11-01), Michnick et al.
Webb et al. (1993) J. Biol. Chem. 268:13994-14002.
Morabito et al. (1991) J. Biol. Chem. 266:9667-72.
Benzinger et al. (1995) Appl. Theroet. Electrophor. 4:161-5.
Tirasophon et al. (1991) Biochem. Biophys. Res. Commun. 175:179-184.
Joyce (1989) Gene 82:83.
Joyce and Inoue (1989) Nucleic Acids Research 17:711.
Ellington and Szostak (1990) Abstract of papers presented at the 1990 meeting on RNA Processing, Cold Spring Harber Laboratory, Cold Spring Harbor, NY, p. 84.
Kinzler and Vogelstein (1989) Nucleic Acids Research 17:3645.
Kramer et al. (1974) J. Mo. Biol. 89:719.
Levisohn and Spiegelman (1969) Proc. Natl. Acad. Sci USA 63:805.
Levisohn and Spiegelman (1968) Proc. Natl. Acad. Sci. USA 60:866.
Oliphant et al. (1989) Mol. Cell. Biol. 9:2944.
Oliphant and Struhl (1988) Nucleic Acids Research 16:7673.
Oliphant and Struhl (1987) Methods in Enzymology 155:568.
Oliphant et al. (1986) Gene 44:177.
Robertson and Joyce (1990) Nature 344:467.
Thiesen and Bach (1990) Nucleic Acids Research 18:3203.
Chen et al. (1993) Biochem. and Biophys. Res. Comm. 191:18-25.
Drolet et al. (1996) Nature Biotech. 14:1021-1025.
Kawazoe et al. (1996) Patterned Staining by Fluorescein-Labeled Oligonucleotides Obtained by In Vitro Selection, Anal. Chem. 68:4309-4311.
Sano et al. (1992) Science 258:120-122.
Drolet Dan
Gold Larry
Jayasena Sumedha D.
Atzel Amy
NeXstar Pharmaceuticals, Inc.
Zitomer Stephanie W.
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