Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1992-11-30
1995-05-23
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
422 56, 422 57, 422 58, 435 792, 435 794, 435810, 436165, 436169, 436524, 436525, 436528, 436530, 436536, 436538, 436540, 436541, 436805, 436807, 436809, 436810, G01N 33537, G01N 33538, G01N 33543, G01N 33553
Patent
active
054181712
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to an analyzer which can easily determine the presence or absence of a target analyte in a sample through immunoassay or the like.
2. Background Art
The presence or absence of a target analyte in a sample has hitherto been determined by bringing the sample into contact with a substance capable of specifically binding to the target analyte and detecting a binding reaction between the target analyte and the substance . Examples of such a technique include in immunological technique by virtue of an antigen-antibody reaction and a hybridization technique for determining the presence or absence of a target sequence through a hybridization with a specific oligonucleotide.
With respect to the immunological technique, various methods are known wherein a liquid sample is brought into contact with a carrier having a surface on which an antibody capable of specifically binding to an antigen has been immobilized and a labelled antibody is allowed to treat the carrier, thereby determining the presence of an antigen in the liquid sample.
For example, Japanese Patent Laid-Open No. 127160/1988 discloses an apparatus wherein a membrane having an antibody bound thereto in a spot form is put on the top surface of a cylindrical container and the lower portion is filled with a highly hygroscopic substance. In this apparatus, at the outset, a liquid sample is added, and an antibody labelled with a gold colloid particle is dropwise added thereto. If the liquid sample contains an antigen in a detectable concentration or more, a reddish purple spot is observed.
Similar apparatuses are disclosed in Japanese Patent Publication Nos. 502214/1986 and 25551/1988. In these apparatuses, it is necessary to conduct two steps, i.e., the step of adding a liquid sample to the membrane portion for allowing the liquid sample to be absorbed into the membrane and the step of dropwise adding a labelled antibody solution. Thus, the procedure may become complicate. Further, in these apparatuses, the membrane easily dries. It is unfavorable for the membrane to easily dry, because this gives rise to a difference in the results between immediately after the reaction and after drying. Further, when the liquid sample is a urine or feces suspension or the like, an apparatus having the surface of the membrane which is exposed to air even after the reaction is unsanitary. Moreover, the apparatus having the surface of the membrane which is exposed to air is unsuitable for transportation.
SUMMARY OF THE INVENTION
An object of the present invention is to provide a simple analyzer.
Another object of the present invention is to provide an apparatus having a surface of a membrane which does not easily dry after a reaction, whereby results of reaction are less susceptible to change with the elapse of time and whereby the apparatus is sanitary and easy to transport.
The analyzer according to the present invention relates to an apparatus for determining the presence or absence of a target analyte in a liquid sample, comprising: transparent portion; and container comprising; has on the main surface a substance capable of specifically binding to the target analyte; and insertion member is inserted into the container, the main surface can be observed from the Outside of the container through the transparent portion of the vessel and the liquid sample is absorbed into the absorbent through the porous member. target analyte and a substance capable specifically binding to the target substance can be easily observed Outside the apparatus.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a sectional view of a preferred embodiment of the analyzer according to the present invention;
FIG. 2 is a sectional view of another preferred embodiment of the analyzer according to the present invention;
FIG. 3(a) is a perspective view of a further preferred embodiment of an analyzer according to the present invention comprising insertion member 2 provided with member 21, said member 21 having a substance
REFERENCES:
patent: 4146365 (1979-03-01), Kay et al.
patent: 4727019 (1988-02-01), Valkirs et al.
patent: 5145789 (1992-09-01), Cortiv et al.
Patent Abstracts of Japan; vol. 12, No. 380 (P-769) 12 Oct. 1988; abstract.
Aoyagi Minoru
Harakawa Kiyomi
Kimura Fumio
Koizumi Naohisa
Matsuo Koichi
Chin Christopher L.
Meiji Seika Kabushiki Kaisha
Scheiner Toni R.
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