Method for detecting a nucleic acid analyte using an improved el

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 435 912, 436544, 436546, 436 94, 436801, 536 243, 536 231, 935 76, 935 77, 935 78, 252700, C12Q 168, C12P 1934, C07H 2104, C12N 1500

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active

056862440

ABSTRACT:
This invention relates to a new electrochemiluminescent (ECL) label for oligonucleotides using phosphoramidite chemistry.

REFERENCES:
patent: 4965188 (1990-10-01), Mullis et al.
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Yee C., Krishnan-Hewlett, I., Baker C.C., Schlegel, R., and Howley, P.M., "Presence and Expression of Human Papillomavirus sequences in human cervical carcinoma cell lines", Am. J. Pathol. 119, 361-6 (1985).
Mullis, K.B., and Faloona,F.A., "Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction", Methods Enzymol 155, 335-50 (1987).
Updyke, T.V. and Nicolson, G.L., "Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and streptavidinagarose", Methods Enzymol. 121, 717-25 (1986).
Cardullo, R.A., Agrawal, S., Flores, C., Zamecnik, D.C., and Wolf, D.E., "Detection of nucleic and hybridization by nonradiative fluorescence resonance energy transfer", Proc. Natl. Acad. Sci. 85, 8790-4 (1988).
Ou, C-Y, Kwok, S., Mithcell, S.W. Mack, D.H., Sninsky, J.J., Krebs, J.W., Feorino, P., Warfield, D., and Schochetman, G., "DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells", Science 239, 295-97 (1988).
Bannworth et al., "A Simple Specific Labelling for Oligonucleotides by Barthophenanthroline. RuII complexes as Nonradioactive Label Molecules", Tetrahedron Letters, vol. 30, No. 12, pp.1513-1516, 1989.
Erhan and Greller, "Do Immunoglobulins have proteolytic activity" Nature, vol. 251, 353-355 (1974).
Y. Weinstein, et al. "Cyclic GMP Stimulates lympphocyte nucleic acid synthesis" Nature, vol. 251, 352-353, (1974).
Sommer et al, "Minimal Homology Requirements for PCR Primers" Nucleic Acids Research, vol. 17, No. 16 p. 6749 (1989).
Rodriguez et al., "Electrochemical Studies of the Interaction of Metal Chelates with DNA. 4. Voltammetric and Electrogenerated Chemiluminescent Studies of the Interaction of Tris (2,2'-bipyridine)osminum (II) with DNA," Anal. Chem., vol. 62 (1990) pp. 2658-2662.

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