Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Reexamination Certificate
2004-08-06
2009-08-04
Ibrahim, Medina A (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
C800S298000, C800S288000, C800S317300, C800S320100, C800S278000, C435S430000, C435S468000, C435S431000, C435S424000, C435S428000
Reexamination Certificate
active
07569746
ABSTRACT:
The invention relates to the use of a modifiedAgrobacteriumto deliver proteins directly to plant cells. Proteins of interest are delivered to the plant host in the form of a fusion protein with theAgrobacteriumvirulence protein VirF. Nucleotide sequences encoding such fusion proteins of VirF and a protein of interest are provided. Also provided are bacteria modified to comprise such fusion proteins of VirF and a protein of interest. Methods of introducing such fusion proteins into a plant host are provided.The invention finds use in facilitating plant transformation and particularly in the bio-engineering of desirable traits into crop plants.
REFERENCES:
patent: 6087175 (2000-07-01), John
patent: 6800791 (2004-10-01), Bailey et al.
patent: WO 98/41642 (1998-09-01), None
Regensburg-Tuink, AJ et al (Nature (1993) 363: 69-71).
Melchers et al (Plant Mol Biol Feb. 1990;14(2):249-59).
Schramejer et al, 2003, Nucleic Acids Research, 31:860-868.
DeVeylder et al., EMBL Database, Accession No. AJ000016 (1997).
DeVeylder et al., “TheArabidopsisCksl At Protein Binds the Cyclin-dependent Kinases Cdc2aAt and Cdc2bAt,”FEBS Letters, 1997, pp. 446-452, vol. 412(3).
Doonan et al., “Conserved and Novel Regulators of the Plant Cell Cycle,”Cell Biology, 1997, pp. 824-830, vol. 9.
Ducommun et al., “Mutations at Sites Involved in Suc1 Binding Inactivate Cdc2,”Mol. Cell. Biol., Dec. 1991, pp. 6177-6184, vol. 11(12).
Herrerra-Estrella et al., “A Bacterial Peptide Acting as a Plant Nuclear Targeting Signal: The Amino-terminal Portion ofAgrobacteriumVirD2 Protein Directs a β-galactosidase Fusion Protein into Tobacco Nuclei,”Proc. Natl. Acad. Sci. USA, Dec. 1990, pp. 9534-9537, vol. 87.
Hindley et al., “Suc1Encodes a Predicted 13-Kilodalton Protein That Is Essential for Cell Viability and Ise Directly Involved in the Division Cycle ofSchizosaccharomyces pombe,” Mol. Cell. Biol., Jan. 1987. pp. 504-511, vol. 7(1).
John et al., “Association of the Plant p34cdc2-like Protein with p13suc1: Implications for Control of Cell Division Cycles in Plants,”Protoplasma, 1991, pp. 70-74, vol. 161.
Melchers et al., “Octopine and Nopaline Strains ofAgrobacterium TumefaciensDiffer in Virulence; Moelcular Characterization of thevirFLocus,”Plant Molecular Biology, 1990, pp. 249-259, vol. 14(2).
Patra et al., “Xc-p9, aXenopusSucl/Cks Homolog, has Multiple Essential Roles in Cell Cycle Control,”Genes&Development, 1996, pp. 1503-1515, vol. 10.
Pines, “Reaching for a Role for the Cks Protein,”Current Biology, 1996, pp. 1399-1402, vol. 6(11).
Regensburg-Tuink et al., “TransgenicN. glaucaPlants Expressing Bacterial Virulence GenevirFare Converted into Hosts for Nopaline Strains ofA. tumefaciens,” Nature, May 6, 1993, pp. 69-71, vol. 363(6424).
Zhang et al., “At the Maizc/AgrobacteriumInterface: Natural Factors Limiting Host Transformation,”Chemistry&Biology, 2000, pp. 611-621, vol. 7(8).
Bailey Matthew A.
Bhattacharjee Saikat
Gelvin Stanton B.
Gordon-Kamm William J.
Lowe Keith S.
Alston & Bird LLP
Ibrahim Medina A
Pioneer Hi-Bred International , Inc.
Purdue Research Foundation
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