Method for delivering oxygen to a cell culture medium

Details Method for delivering oxygen to a cell culture medium Method for delivering oxygen to a cell culture medium

1992-11-05

1994-11-15

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524025, 435818, C12N 500

Patent

active

053647840

ABSTRACT:
An apparatus and method for delivering oxygen to a cell culture medium is disclosed. In one embodiment, the apparatus includes a transfer chamber placed within the cell culture medium. The transfer chamber is adapted to allow the cell culture medium to come in substantial contact with a liquid chemical that is passing through transfer chamber. The apparatus further includes a vessel placed outside the cell culture medium that serves to contain and continually recharge the liquid chemical. The apparatus further includes a plurality of pumps and tubes for circulating the liquid chemical through the transfer chamber and back to the vessel. When the liquid chemical comes into contact with the cell culture medium, oxygen is transferred from the liquid chemical to the cell culture medium.

REFERENCES:
patent: 3821087 (1974-06-01), Knazek et al.
patent: 4220725 (1980-07-01), Knazek et al.
patent: 4720462 (1988-01-01), Rosenson
patent: 4749654 (1988-06-01), Karrer et al.

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