Method for culturing mesenchymal stem cell and method for...

Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...

Reexamination Certificate

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C435S001100, C435S325000

Reexamination Certificate

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07972767

ABSTRACT:
The purpose is to proliferate a mesenchymal stem cell to a sufficient degree while reducing the amount of blood serum contained in a biological tissue progenitor cell to be grafted, and to efficiently differentiate the mesenchymal stem cell into the biological tissue progenitor cell. There is provided a method for culturing a mesenchymal stem cell, comprising: a first culture step of proliferating a mesenchymal stem cell in a medium containing blood serum; and a second culture step of differentiating the mesenchymal stem cell into a biological tissue progenitor cell in a medium containing blood serum at a lower concentration than that in the medium used in the first culture step.

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Date-of-Receipt letter to establish the date (Feb. 15, 2010) on which the Search Report was received.
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European Office Action dated Jun. 10, 2010, issued in European Application No. 06746151.7.
Extended European Search Report dated Dec. 30, 2009 of corresponding European Application No. 09014695.2.

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