Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1994-12-22
1995-08-22
Knode, Marian C.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352403, 43524021, 424529, 424531, C12N 500, A61K 3556, A61K 3560
Patent
active
054439841
ABSTRACT:
A method for culturing mammalian cells using fish serum. The method uses serum extracted from the blood of fishes to culture mammalian cells for various purposes. The technique has the key advantages of 1) freedom from mammalian infectious agents that could contaminate cell lines or endanger researchers or recipients of therapeutants derived from mammalian cell culture; 2) consistent and reproducible serum content and quality; 3) low cross-reactivity; and 4) provision of the appropriate serum nutrients to maintain the growth of mammalian cells. Fish serum is used together with designated defined media to allow mammalian cells to grow and populations of these cells to be maintained. Blood serum is derived from captive stocks of fish raised under controlled conditions such that the diet, habitat, genetics, life history, and reproductive status of the fish remains substantially consistent and reproducible.
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Sawyer Evelyn S.
Sawyer Philip J.
Champagne Thomas M.
Dadio Susan M.
Knode Marian C.
Roberts John L.
Sea Run Holdings, Inc.
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