Method for culturing insect cells in a medium containing fish se

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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43524021, 4352401, 424531, C12N 500, A61K 3556, A61K 3560

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054985404

ABSTRACT:
A method for culturing insect cells using fish serum. The method uses serum extracted from the blood of fish to culture insect cells for various purposes. The technique has the key advantages of consistent quality, low cross reactivity, safety from infectious agents that would endanger researchers or humans and animals receiving cell culture products as therapy and has appropriate nutrients to maintain the growth of insect cells. Fish serum is used together with designated defined medium to allow insect cells to grow and populations to be maintained. The method may also be used with lumpfish to prevent the attachment of insect cells to cultureware. Serum is derived from the blood of captive stocks of fishes raised under control conditions.

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Parasitol Res., vol. 77, No. 8, 1991, Hamers et al, "In Vitro Study of the Impact of Fish Sera on the Survival and Fine Structure of the Eel-Pathogenic Acanthocephalan Paratenuisentis Ambiguus", pp. 703-708, see entire document.
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"Manufacturers Warned Not to Use Bovine Origin Materials From BSE Countries" FDA Veterinarian, Mar./Apr. 1994.

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