Method for culturing human hematopoietic stem cells in vitro

Details Method for culturing human hematopoietic stem cells in vitro Method for culturing human hematopoietic stem cells in vitro

1992-04-03

1995-07-25

Lacey, David L.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352402, 43524021, 435240241, 43524025, 435284, C12N 502, C12N 508

Patent

active

054361516

ABSTRACT:
A method for culturing mammalian hematopoietic stem cells is provided comprising maintaining a population of human stem cells in fixed, non-contacting relationship to a population of cultured stromal cells, which populations are in liquid stromal growth medium connection, so that the ability of the stem cells to differentiate and self-replicate is maintained during an extended culture period.

REFERENCES:
patent: 4342828 (1982-08-01), Takaku et al.
patent: 4714680 (1987-12-01), Civin
patent: 4721096 (1988-01-01), Naughton et al.
patent: 4808611 (1989-02-01), Cosman
patent: 4873191 (1989-10-01), Wagner et al.
patent: 4902783 (1990-02-01), Goda et al.
patent: 4946437 (1990-08-01), Sredni et al.
patent: 4965204 (1990-10-01), Civin
patent: 5004681 (1991-04-01), Boyse et al.
patent: 5035994 (1991-07-01), Civin
patent: 5061620 (1991-10-01), Tsukamoto et al.
patent: 5104804 (1992-04-01), Humphries et al.
patent: 5139951 (1992-08-01), Butz et al.
Verfaillie et al. Blood 82:2045-2053(1993).
Verfaillie et al. Blood 79:2821-2826(1992).
Groenberger in Hematopoiesis (ED. D. W. Golde) pp. 203-242 Churchill Livingstone N.Y. 1984.
H. J. Sutherland et al., Blood, 78, 666 (1991).
C. Smith et al., Blood, 77, 2122 (May 15, 1991).
M. A. S. Moore, Blood, 78, 1 (Jul. 1, 1991).
M. A. S. Moore, Cancer Supp., 2718 (May 15, 1991).
R. M. Schwartz, PNAS USA, 88, 6760 (Aug., 1991).
E. F. Srour et al., Blood Cells, 17, 287 (1991).
C. C. Fraser et al., PNAS USA, 89, 1968 (Mar. 1992).
C. M. Baum et al, PNAS USA, 89, 2804 (Apr. 1992).
Costar "Transell" Tech Data Sheet (no date).
H. J. Sutherland et al., PNAS USA, 87, 2584 (1990).
J. Brandt et al., J. Clin. Invest., 86, 932 (Sep. 1990).
B. D. Luskey et al., Annal. N.Y. Acad. Sci., 612 398 (1990).
J. Caldwell et al., J. Cell. Physiol., 147, 344 (1991).
L. W. M. M. Terstappen et al., Blood, 77, 1218 (Mar. 1991).
R. M. Lemoli et al., Blood, 77, 1829 (Apr. 15, 1991).
E. Bruno et al., Blood, 77, 2339 (Jun. 1991).
J.-H. Shieh et al., J. Immunol., 147, 2984 (1991).
C. M. Verfaillie et al., Blood, 77, 263 (1991).
S. J. Szilvassy, PNAS USA, 86, 8798 (1989).
H. J. Sutherland et al., Blood, 74, 1563 (1989).
C. Verfaillie et al., J. Exp. Med., 172, 509 (1990).
M. A. S. Moore, Cancer Surveys, 9, 7 (1990).
D. M. Bodine, Ann. N.Y. Acad. Sci., 612, 415 (1990).
D. Zipori et al., Exp. Hematol., 8, 816 (1990).
P. M. Lansdorp et al., J. Exp. Med., 172, 363 (1990).
R. G. Andrews et al., J. Exp. Med., 172, 355 (1990).
H. H. Gerhartz et al., Blood, 76, 274a, Abstract 1087 (1990).
W. W. Kwok et al., PNAS USA, 83, 4552 (1986).
I. R. Freshney, "Hemopoeitic Cells," in Culture of Animal Cells, A. R. Liss, N.Y. (187) at pp. 284-288.
M. Y. Gordon et al., J. Cell. Physiol., 130, 150 (1987).
M. Y. Gordon et al., Nature, 326, 403 (1987).
T. Friedmann, Lancet, 1271 (Jun. 4, 1988).
G. J. Spangrude et al., Science, 241, 58 (1988).
J. Brandt et al., J. Clin. Invest., 82, 1017 (1988).
T. Friedmann, Science, 244, 1275 (1989).
I. Bertoncello et al., Exp. Hematol., 17, 171 (1989).
T. M. Dexter et al., J. Exp. Med., 145, 1612 (1977).
S. Gartner et al., PNAS USA, 77, 4756 (1980).
I. R. G. Toogood et al., Leukemia Res., 4, 449 (1980).
S. A. Bentley, Exp. Hematol., 9, 308 (1981).
F. T. Slovick et al., Exp. Hematol., 12, 327 (1984).
M. Y. Gordon et al., Internat. J. Cell Cloning, 1, 429 (1983).
L. Simonovitch, J. Cell. Comp. Physiol., 64 23 (1964).
T. M. Dexter et al., J. Cell. Physiol., 91, 335 (1977).
R. Shields et al., J. Cell. Physiol., 91 345 (1977).

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