Method for culturing cells or tissues

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including perfusion; composition therefor

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435240, 435241, 128 1, A01N 102

Patent

active

045088195

ABSTRACT:
Disclosed herein is a process for the in vitro culturing of cells or tissues such that the cells or tissues grow, behave and function in substantially the manner such cells or tissues grow, behave and function within the body, wherein the cells or tissues are contacted or bathed with fresh flowing cell-free lymph. The cell-free lymph may be obtained directly from a living host by removing lymph from the host, rendering the lymph cell-free by suitable means and then using the cell-free lymph as the culture medium for cells or tissues of either the same or different species as that of the host. The cells or tissues preferably are contacted with the cell-free lymph in a chamber in which the cells or tissues are exposed either directly to the flowing lymph or to components thereof.

REFERENCES:
Willmer-Cells & Tissues in Culture, vol. 2, (1965), pp. 373-374.
Chang-Artificial Cells (1972) Thomas, publisher, pp. 3-14, 113-132.

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