Method for culturing bordetella pertussis, a pertussis toxoid an

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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4352521, 4352525, 435244, 435248, 435243, 435 68, 435832, 424 88, 424 92, C12N 122

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active

048493589

ABSTRACT:
There is disclosed a method for culturing Bordetella pertussis in the prece of a cellulose and/or cellulose derivatives. The present method is useful for obtaining a mixed antigen comprising pertussis toxin and filamentous hemagglutinin in a large amount at low cost. From the antigen, there can be obtained a stable and effective pertussis toxoid to be used for a pertussis vaccine. There is also disclosed a vaccine comprising the pertussis toxoid as an active ingredient and a gelatin and/or gelatin derivatives as a stabilizing agent. The present vaccine is extremely stable and can be stored for a prolonged period of time.

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