Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Patent
1988-12-19
1992-04-07
Lacey, David L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
4352402, 43524023, 435283, 435284, 435300, C12P 2100, C12M 300, C12M 114
Patent
active
051027909
ABSTRACT:
A method of using an apparatus for the in vitro cultrue of animal cells to recover secreted proteins of interest therefrom, affording the capability of being readily and predictably scaled up or down to desired protein productin rates, comprised of a housed arrangement of culture subunits containing a packed bed of carrier particles, each subunit being fed in parallel from a common culture medium source, and the spent culture fluid from each subunit being directed to a common spent fluid conduit within the housed arrangement. Scale-up or scale-down is effected by addition or subtraction of culture subunits and/or by increase or decrease in carrier particle volume within a subunit while maintaining substantially constant packed bed height.
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Bliem Rudolf F.
Oakley Robert V.
Taiariol Van C.
Baxter International Inc.
Beisner William H.
Lacey David L.
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