Method for culturing and floating animal cells in a double-bag c

Details Method for culturing and floating animal cells in a double-bag c Method for culturing and floating animal cells in a double-bag c

1991-02-14

1991-12-10

Lacey, David L.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352401, 435240241, C12N 506, C12N 502

Patent

active

050717600

ABSTRACT:
The instant invention relates to a method for culturing and floating animal cells in a double-bag container, where an outer bag containing culture media and a volume of air houses an inner bag of a semipermeable film containing cells suspended in a culture media. The semipermeable film has pores of sufficient size so as to prohibit cells within the inner bag from passing therethrough but allowing culture liquid and air to pass through it. To optimize the exchange of nutrients between the interiors of the two bags, a protective mesh surrounds the inner bag and the double-bag container is fastened to an agitator which provides either a rotating or shaking motion.

REFERENCES:
patent: 2494866 (1950-01-01), Fressola
patent: 4183677 (1980-01-01), de Bruyne
patent: 4293643 (1981-10-01), Ohtake et al.
patent: 4296205 (1981-10-01), Verma
patent: 4661455 (1987-04-01), Hubbard

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