Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2002-09-16
2009-12-22
Lilling, Herbert J. (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C435S286500, C435S287100, C435S297200, C435S297500, C435S305300, C435S325000, C435S366000
Reexamination Certificate
active
07635575
ABSTRACT:
The invention relates to a method for cultivating cells of the most diverse type, particularly human or animal cells. According to the invention, a culture is prepared from cells of at least one specified type in a defined environment, and the cells of the relevant culture are supplied with assigned, liquid nutrient media, growth factors, gases and the like. A combination of the following method steps is provided: a) preparing at least one cell culture inside at least one cell culture chamber (20) of a cell culture system (30); b) starting a flow of freely selectable, liquid media into the at least one cell culture chamber (20) in order to continuously supply the at least one cell culture; c) starting a flow of different gases with freely selectable concentrations into the at least one cell culture chamber (20) in order to effect a constant and continuous gassing of the at least one cell culture; d) effecting a regulated or controlled heating of the at least one cell culture chamber (20) in such a manner as to ensure a constant temperature therein over the duration of an experiment; e) carrying out permanent microscopic observation of the at least one cell culture inside the at least one cell culture chamber (20) without removing samples of the cell culture over the duration of an experiment, and; f) permanently measuring all relevant cell culture parameters using corresponding sensors that are integrated inside the at least one cell culture chamber (20).
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Scherze Wilhelm
Seidl Josef
Lilling Herbert J.
PAN-Biotech GmbH
Schwegman Lundberg & Woessner, P.A.
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