Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of co-culturing cells
Reexamination Certificate
2008-07-01
2008-07-01
Canella, Karen A. (Department: 1643)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of co-culturing cells
C435S374000, C435S378000, C435S379000, C435S408000, C436S064000
Reexamination Certificate
active
09914662
ABSTRACT:
The invention relates to a method for cultivating cancer cells for scientific serial assays, wherein a tissue sample which is heterogeneous with respect to contaminants, normal cells and tumor cells is locally separated in a sequential-parallel splitting method. The locally separated sample segments are further split, wherein the tissue fragments and liquids of the tissue segments are separately placed in a given cell culture medium and grown under predetermined culture conditions. The invention also relates to a cell culture medium and a device for splitting the tissue samples into disc segments. The inventive method combined with the splitting device and the culture medium enables fast cultivation of cancer cells obtained from human tissue with a multiplication rate of 100% in all types of tumors.
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Canella Karen A.
MagForce Applications GmbH
Wood Phillips Katz Clark & Mortimer
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