Method for crystallization of enzymes

Details Method for crystallization of enzymes Method for crystallization of enzymes

1995-06-07

1998-11-17

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435221, 435814, 435816, C12N 900

Patent

active

058375139

ABSTRACT:
A method for rapidly and inexpensively crystallizing enzymes from an impure mixture is disclosed. The yield of the enzyme is greater than 35% within twelve hours. The crystallizing agent is a salt added in a concentration which is less than the concentration necessary to crystallize the enzyme in amorphous form.

REFERENCES:
patent: 3623957 (1971-11-01), Feldman
patent: 5041377 (1991-08-01), Decker et al.
Scopes, R.K Protein Purification; Pinciples and Practice, Second Edition, New York, Springer-Verlag, 1987, pp. 41-54. 182!, Academic Press, 1990, pp. 285-300.
McPherson, Preparation and Analysis of Protein Crystals (1989).
Steinrauf, Acta Crystallographica, vol. 12, pp. 77-79 (1959).
Melis, et al., The Journal of Biological Chemistry, vol. 258, No. 10, pp. 6255-6257 (1983).
Swan, Journal of Molecular Biology, vol. 60, No. 2, pp. 405-407 (1971).
Sheriff, et al., Journal of Molecular Biology, vol. 145, No. 2, pp. 441-451 (1981).

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