Refrigeration – Processes – Treating an article
Patent
1987-01-02
1990-01-02
Richman, Barry S.
Refrigeration
Processes
Treating an article
435 1, 435 2, 128DIG27, F25D 1304
Patent
active
048904570
ABSTRACT:
A method of freezing, storing and thawing collagen-rich tissue, such as heart valves. The method includes a freezing profile for freezing the tissue down to the temperature of liquid nitrogen with minimal tissue damage due to ice crystal formation. Heart valves cryopreserved according to the present process exhibit a high cell viability when thawed and are suitable for implanting in human patients.
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An Organic Cryopreservation Apparatus, Michael G. O'Callaghan et al., IEEE Transactions on Biomedical Engineering, vol. BME-24, No. 2 (Mar. 1977).
Preservation of Aortic Heart Valves with Maintenance of Cell Viability, Arthur W. M. Van der Kamp, M.D. et al., Journal of Surgical Research 30, 45-56 (1981).
Bank Harvey L.
Brockbank Kelvin G. M.
Heacox Albert
McNally Robert T.
Cryolife, Inc.
Richman Barry S.
Wallen T. J.
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