Method for cryopreserving blood vessels

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including perfusion; composition therefor

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62 64, A01N 102

Patent

active

051588676

ABSTRACT:
A device for use in cryopreservation of blood vessels comprising a pair of stylets insertable into the ends of a dissected blood vessel segment. The stylets are mountable on a support track whereby the blood vessel can be distended and supported during cryopreservation procedures. Also disclosed is a freezing and thawing profile capable of maximizing endothelial cell survival. The use of chondroitin sulfate or similar compound is discussed as a novel cryoprotectant.

REFERENCES:
patent: 4959319 (1990-09-01), Skelnik et al.
Sachs et al., Endothelial Integrity after Venous Cryopreservation, Journal of Surgical Research 32, 218-227 (1982).

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