Method for cryopreservation of primordial germ cells and germ ce

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...

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435 2, 4352402, 424561, 600 34, A01N 102, C12N 500

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057597634

ABSTRACT:
Cryopreservation of primordial germ cells and germ cells, in which primordial germ cells or germ cells are separated from bird embryos before meiosis and are then suspended and frozen in a freezing medium containing a cryoprotective agent is useful for the conservation of species of birds, for the protection of rare species which are in danger of extinction and also for the production of transgenic animals, the production of novel animals having gene markers on a cellular level, the production of animals useful as disease models, the production of useful substances by genetic engineering, and the development of test methods for toxicity to germ-line cells.

REFERENCES:
patent: 5342776 (1994-08-01), Bolnet
Molecular Reproduction and Development, vol. 39, No. 2, pp. 153-161, Oct. 1, 1994, Mitsuru Naito, et al., "Production of Germline Chimeric Chickens, with High Transmission Rate of Donor-Derived Gametes, Produced by Transfer of Primodial Germ Cells".
British Poultry Science, vol. 33, pp. 449-453, 1992, M. Naito, et al., "Preservation of Quail Blastoderm Cells in Liquid Nitrogen".
Theriogenology, vol. 33, No. 1, p. 272, Jan. 1990, F. Leichthammer, et al., "In Vitro Culture and Cryopreservation of Farm Animals' Primodial Germ Cells".
Theriogenology, vol. 40, pp. 509-519, 1993, A. Tajima, et al., "Production of Germ Line Chimera by Transfer of Primordial Germ Cells in the Domestic Chicken (Gallus Domesticus)".
Tajima A., Production of Germ Line Chimera by Transfer of Primordial Germ Cells in the Domestic Chicken, Theriogenology 40: 509-519.
Naito M., Production of Germline Chimeric Chickens, With High Transmission Rate of Donor-Derived Gametes, Produced by Transfer of Primoridal Germ Cells, Molecular Reproduction and Development 39:153-161.
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V. Hamburger et al, Developmental Dynamics, vo. 195, pp.231-272 (1992).
Leibo S., Techniques for Preservation of mammalian Germ Plasm, Animal Biotech 3(1) 139-153 (1992).
Petitte J., Accessing the Genome of the Chicken . . . Manipulation of the Avian Genome CRC Press pp. 81-101 1993.
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Subramoniam T., Cryopreservation of Crustacean . . . Proc Indian Natn Sci Acad B60(3) pp. 229-236 1994.

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