Method for cryopreservation of biological samples

Details Method for cryopreservation of biological samples Method for cryopreservation of biological samples

1996-11-01

1998-02-10

Capossela, Ronald C.

Refrigeration

Storage of solidified or liquified gas

Cryogen stored in both phases

62 78, 62100, F17C 500

Patent

active

057156867

ABSTRACT:
A method for cryopreservation of biological samples. A cryogenic liquid such as liquid nitrogen is turned into a slush within a container such as a standard cryogenic storage vial by applying suction to create a partial vacuum above the liquid. The sample to be cooled is dropped into the slush and is subsequently stored in the container within which it was cooled.

REFERENCES:
patent: 3994141 (1976-11-01), Schrawer
patent: 4302950 (1981-12-01), Sitte
patent: 4827737 (1989-05-01), Oda et al.
patent: 4840034 (1989-06-01), Liberman
Mazur et al: "Cryobiological Preservation of Drosophila Embryos"; Science, vol. 258, pp. 1932-1935 (1992).

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