Refrigeration – Processes – Treating an article
Patent
1986-11-04
1989-01-24
Lusignan, Michael R.
Refrigeration
Processes
Treating an article
34 5, 62 78, 435 1, F24F 316, F25D 1702
Patent
active
047993618
ABSTRACT:
This invention relates to apparatus for the cryopreparation of biological tissue samples for ultrastructural analysis. The use of apparatus comprises vitrifying a biological tissue sample under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a sample holder at a temperature of less than -140.degree. C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is optionally infiltrated with a degassed resin followed by a polymerization of the resin to form an embedded tissue sample.
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Board of Regents , The University of Texas System
Lusignan Michael R.
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