Method for cryopreparing biological tissue

Details Method for cryopreparing biological tissue Method for cryopreparing biological tissue

1988-07-11

1989-09-12

Lusignan, Michael

Coating processes

Plant member or animal specimen coating

34 5, 62 64, 62 78, 427250, 435 1, A01G 506, A01N 100, A01N 300

Patent

active

048658715

ABSTRACT:
This invention relates to apparatus for the cryopreparation of biological tissue samples. The method comprises treating a biological tissue sample with a cryoprotectant which raises the glass transition temperature range of the sample followed by vitrification under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a sample holder. The tissue sample is then dehydrated while maintained in a state of thermal nonequilibrium.

REFERENCES:
patent: 4423600 (1984-01-01), McKenna
patent: 4559298 (1985-12-01), Fahy
patent: 4688387 (1987-08-01), Conaway

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